Objective To Investigate the human Brucella infection status and brucellosis epidemic characteristics in Kuitun and its surrounding areas from 2014 to 2016, so as to provide the evidence for prevention and control of human brucellosis.Methods According to WS269-2007 "Brucellosis Diagnosis Standard", the occupational groups and close contacts were investigated.Results During the period of 2014 to 2016, the positive rate of Brucella specific sero-antibody in Kuitun and its surrounding areas was the highest in 2015. Compared with the positive rate of 2015, the positive rates in 2014 and 2016 had significant differences (X2 values were 6.99 and 67.10 respectively, both P＜0.01). There was no significant difference in the positive rate between year 2014 and 2016 (X2=0.82, P＞0.05). There was no significant difference between Han nationality and Kazak nationality in the positive rate. Among the age groups, the positive rate was the highest in the 21-30 years group (27.40%) and lowest in the 71-80 years group (5.45%), and there was a significant difference avmong the age groups (X2=55.67, P＜0.01). In the persons with Brucella infection, the specific antibody positive rate of the male was 27.76% and the positive rate of the female was 10.71%, and there was a significant difference between them (X2=102.72, P＜0.01). As the occupational distribution, the infection rate of herdsmen was the highest (45.39%), followed by farmers (26.74%). As the regional distribution, Boertong ancient pasture of Shawan County had the highest infection proportion (34.61%), followed by Kuitun urban area (13.48%). Conclusion The human Brucella infection in Kuitun and its surrounding areas cannot be ignored. It is necessary to pay much attention to the monitoring and prevention of human brucellosis, and also it is necessary to take effective interventions, including targeted health education, etc. especially in the high-risk population.